The electric fan is good for highway mpg since it's freeing up some horsepower, and the highway airflow is more than enough to cool the radiator. But in the city, the electric fan will run a lot, and with the a/c on you have to have the fan on no matter what- unless your above 50 mph. So overall the electric fan will help hwy mpg, but not much in the city. (I know from experience)
TB spacer--waste of $(from experience too...)
Cold air intake.....the cooler air requires more fuel to keep the same air:fuel ratio, so CAI = worse mpg. However, improving the airflow after the filter to the throttle body can help the efficiency and improve mpg....For instance replacing the stock piping with one that's smoother flowing will help.
E3 plugs? not sure
Cat back is a good start. My goal is headers, high flow cats, throw away the Y pipe and run both pipes into a 2 in/1 out muffler, with a 3" outlet, 2 1/4 inlets. The factory pipes are horribly bent especially at the "y' portion of it.
Custom tune is always a good mod both for power and economy.
Underdrive pulleys are a simple way to gain power through efficiency, which means better mpg.
If you can find places that sell "Boat gas" or "offroad gas" then you'll definitely gain mpg. That fuel does not have ethanol. Ethanol basically waters down our fuel by 10%, and provides very little actual energy. It is higher octane by itself, but no energy. One gallon of ethanol fuel gives 10% less energy than real fuel. I have a local place I get 90 octane off road fuel, and I get 60 miles extra out of one tank of fuel. Not sure the % gained, but it's about 3 1/2 gallons saved in driving.